个人简述:

博士、副研究员、硕士生导师，浙江理工大学生命科学学院教师。

1995~1999年，华中农业大学畜牧兽医学院兽医专业，学士学位；

1999~2006年，浙江大学生命科学学院生物化学与分子生物学专业，理学博士学位。

2006年至今，浙江理工大学生命科学学院教师。

现于浙江理工大学生命科学学院从事科研和教学工作，主讲：基因工程、基因工程实验、核酸技术和蛋白质技术等本科生和研究生课程。科研工作主要包括以昆虫细胞、幼虫或蛹作为生物反应器制备药用蛋白、开发新型的家蚕杆状病毒载体表达系统、杆状病毒分子生物学以及开展家蚕基因功能等方面的研究。目前,完成1项国家“863”课题，主持完成浙江省自然科学基金项目2项，完成浙江省科技厅公益项目1项，参与2项国家自然科学基金项目。已经发表SCI论文20余篇。以第一发明人申报4项国家发明专利，已获1项授权，另有3项公开。

科研工作:

发表论文：

陈和、陈琴、梅文枫、钱月忠、聂作明、于威、陈健、张耀洲. 一种红色荧光蛋白基因稳定转化的家蚕细胞株构建及应用. 蚕业科学，2013，39（3）：0494-0500.

费伟强、陈琴、陈倩、梅文枫、边腾飞、吕正兵、于威、陈健、张耀洲、吴祥甫. 复制缺陷型BmNPV载体的构建及应用. 2013，39（3）：0501-0508.

陈琴、杨丹、聂作明、陈健、张耀洲. 家蚕脂蛋白基因BmLp-c21的表达及亚细胞定位. 蚕业科学，2012，38（3）：0434-0441.

Chen J, Nie ZM, Lv ZB, Zhu CG, Xu CZ, Jin YF, Wu XF, Zhang YZ. Large-Scale Purification of hGM-CSF Expressed in Bombyx mori Pupae. Applied Biochemistry and Biotechnology. 2007 Apr141(1):149-160.

Chen J, Liu T, Zhu CG, Jin YF, Zhang YZ. Genetic variation of Chinese PRRSV Strains based on ORF5 sequence. Biochemical Genetics. 2006 Oct, 44(9-10): 425-435.

Chen J, Wu XF, Zhang YZ. Expression, purification and characterization of human GM-CSF using silkworm pupae (Bombyx mori) as a bioreactor. Journal of Biotechnology. 2006 May 17 123(2): 236-247.

Liu Y, Chen Y, Chen J, Zhang W, Sheng Q,Chen J, Yu W, Nie Z, Zhang Y, Wu W, Wang L, Indran IR, Li J, Qian L, Lv Z. A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein. Mar Drugs. 2013, 11(5): 1492-1505.

Yu W, Du CY, Quan YP, Nie ZM,Chen J, Lv ZB, Zhang YZ. Characterization of late gene expression factor LEF-10 from Bombyx mori nucleopolyhedrovirus. Virus Res. 2013, 175(1): 45-51.

Yu W, Li J, Wang M, Quan Y,Chen J, Nie Z, Lv Z, Zhang Y. The screening and functional study of proteins binding with the BmNPV polyhedrin promoter. Virol J. 2012, 9: 90.

Lan H, Nie Z, Liu Y, Lv Z, Liu Y, Quan Y,Chen J, Zhen Q, Chen Q, Wang D, Sheng Q, Yu W, Chen J, Wu X, Zhang Y. In Vivo Bioassay of Recombinant Human Growth Hormone Synthesized in B. mori Pupae. J Biomed Biotechnol. 20102010:306462.

Zeng F, Xie H, Nie Z,Chen J, Lv Z, Chen J, Wang D, Liu L, Yu W, Sheng Q, Wu X, Zhang Y. Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the Silkworm, Bombyx mori. Comp Funct Genomics. 2009:947490.

Nie Z, Lv Z, Qian J,Chen J, Li S, Sheng Q, Wang D, Shen H, Yu W, Wu X, Zhang Y. Molecular cloning and expression characterization of translationally controlled tumor protein in silkworm pupae. Mol Biol Rep. 2009 Sep 15.

Shen Y, Zhang T, Chen J, Lv Z,Chen J, Wang D, Nie Z, He P, Wang J, Zheng Q, Sheng Q, Wu X, Zhang Y. Molecular Characterization and Tissue Localization of an F-Box Only Protein from Silkworm, Bombyx mori. Comp Funct Genomics. 2009:416040.

Zhang Y, Xia Q, Xu J,Chen J, Nie Z, Wang D, Zhang W, Chen J, Zheng Q, Chen Q, Kong L, Ren X, Wang J, Lv Z, Yu W, Jiang C, Liu L, Sheng Q, Jin Y, Wu X. Aligning the proteome and genome of the silkworm, Bombyx mori. Funct Integr Genomics. 2009 Nov9(4):447-54.

Jin R, Lv Z, Chen Q, Quan Y, Zhang H, Li S, Chen G, Zheng Q, Jin L, Wu X,Chen J, Zhang Y. Safety and immunogenicity of H5N1 influenza vaccine based on baculovirus surface display system of Bombyx mori. PLoS One. 20083(12):e3933.

Nie Z, Zhou R,Chen J, Wang D, Lv Z, He P, Wang X, Shen H, Wu X, Zhang Y. Subcellular localization and RNA interference of an RNA methyltransferase gene from silkworm, bombyx mori. Comp Funct Genomics. 2008:571023.

He PA, Nie Z,Chen J, Chen J, Lv Z, Sheng Q, Zhou S, Gao X, Kong L, Wu X, Jin Y, Zhang Y. Identification and characteristics of microRNAs from Bombyx mori. BMC Genomics. 2008 May 289:248.

Zhang Y, Lv Z,Chen J, Chen Q, Quan Y, Kong L, Zhang H, Li S, Zheng Q, Chen J, Nie Z, Wang J, Jin Y, Wu X. A novel method for isolation of membrane proteins: A baculovirus surface display system. Proteomics. 2008 Sep 228(20):4178-4185.

Nie Z, Xu J,Chen J, Lv Z, Wang D, Sheng Q, Wu Y, Wang X, Wu X, Zhang Y. Expression Analysis and Characteristics of Profilin Gene from Silkworm, Bombyx mori. Appl Biochem Biotechnol. 2008,158(1):59-71.

Jianqing Chen,Jian Chen, Qijing Gai, Zhengbing Lv, Dan Wang, Zuoming Nie, Jiang Wang, Xuedong Wang, Xiangfu Wu, Yaozhou Zhang. Molecular Characterization and Immunohistochemical Localization of a Novel Troponin C During Silkworm Development. Cell Tissue Res. 2008 Mar331(3):725-738.

Lingyin Kong, Zhengbing Lv,Jian Chen, Zuoming Nie, Dan Wang, Hongdan Shen, Xuedong Wang, Xiangfu Wu, Yaozhou Zhang. Expression analysis and tissue distribution of two 14-3-3 proteins in silkworm (Bombyx mori). BBA-General subject, 2007 Dec1770(12):1598-1604.

Wang X,Chen J, Lv Z, Nie Z, Wang D, Shen H, Wang X, Wu X, Zhang Y. Expression and Functional Analysis of CRABP from Silkworm Pupae (Bombyx mori). Journal of Cellular Biochemistry, 2007 Nov 1 102(4): 970-997.

Nie ZM, Zhang ZF, Wang D, He PA, Jiang CY, Song L, Chen F, Xu J, Yang L, Yu LL,Chen J, Lv ZB, Lu JJ, Wu XF, Zhang YZ. Complete sequence and organization of Antheraea pernyi nucleopolyhedrovirus, a dr-rich baculovirus. BMC Genomics. 2007 Jul 24 8: 248.

Zhang YZ,Chen J, Nie ZM, Lu ZB, Wang D, Jiang CY, He PA, Liu LL, Lou YL, Song L, Wu XF. Expression of open reading frames in silkworm pupal cDNA library. Applied Biochemistry and Biotechnology, 2007 Mar136(3):327-343.

Zheng QL,Chen J, Nie ZM, Lv ZB, Wang D, Zhang YZ. Expression, purification and characterization of a three-domain Kazal-type inhibitor from silkworm pupae (Bombyx mori). Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 2007 Feb, 146(2): 234-240. (SCI, IF: 1.404)

Zhang Y,Chen J, Lv Z, Nie Z, Zhang X, Wu X. Can 29kD rhGM-CSF expressed by Silkworm pupae bioreactor bring into effect as active cytokine through Orally Administration? European Journal of Pharmaceutical Sciences. 2006 Jun, 28(3): 212-223. (SCI, IF: 2.482)

Yu W,Chen J, Zhao XL. Expression of polyhedrin-hEGF fusion protein in cultured cells and larvae of Bombyx mori. African Journal of Biotechnology. 2006 5 (11): 1034~1040 (SCI).

夏学春，陈健，张耀洲。人乙肝病毒前表面抗原preS1在家蚕培养细胞中的表达。蚕业科学，2005，31（1）：103-106.

授权专利：可线性化穿梭载体BmBacmid的构建方法；申请号：201210037290.8。